antibodies against ampkα, phosphorylated (p)ampkα (thr172), acc, p-acc (ser79), and β-actin  (Cell Signaling Technology Inc)

Journal: Nutrients

Article Title: Arene Substitutions in Orchid Bibenzyls: Mechanistic Insights into Glucose Uptake and Lipid Metabolism for Targeting Metabolic Disorders

doi: 10.3390/nu17071104

Figure Lengend Snippet: Impacts of batatasin III and gigantol on adipogenesis-mediated signaling pathways. The activation (phosphorylation denoted with prefix p-) levels of some signaling pathways (AMPK-ACC and AKT/GSK-3 β) triggered during early differentiation of 3T3-L1 cells treated with non-cytotoxic concentrations (5–25 μM) of batatasin III ( A – F ) or gigantol ( G – L ) for 48 h were assessed using Western blot analysis. Undifferentiated (UC) and differentiated (DC) cells treated with vehicle (0.5% ( v / v ) dimethyl sulfoxide) served as controls. Protein levels were quantified using ImageJ software and normalized to β-actin. Numerical data are presented as means ± SDs from three independent experiments. Lowercase letters indicate statistically significant differences among means within the same experimental condition. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test at α = 0.05.

Article Snippet: The primary antibodies included GLUT1, GLUT4, PPARγ, C/EBPα, FAS, FABP4, PLIN1, adiponectin, AKT, phosphorylated AKT (p-AKT; Ser473), AMPKα, phosphorylated AMPKα (p-AMPKα; Thr172), AMPKβ1/2, phosphorylated AMPKβ1 (p-AMPKβ1; Ser182), ACC, phosphorylated ACC (p-ACC; Ser79), GSK3β, phosphorylated GSK3β (p-GSK3β; Ser9), and β-Actin, all purchased from Cell Signaling Technology (Danvers, MA, USA), while LPL and SREBP-1c antibodies were sourced from Invitrogen (Waltham, MA, USA).

Techniques: Protein-Protein interactions, Activation Assay, Phospho-proteomics, Western Blot, Software

Journal: Animal Nutrition

Article Title: Different types of bile acids exhibit opposite regulatory effects on lipid metabolism in finishing pigs through bile acid receptors

doi: 10.1016/j.aninu.2024.12.003

Figure Lengend Snippet: Effects of HDCA and LCA on the levels of lipid metabolism-related gene expression in backfat, abdominal fat and liver. Values with different letters mean significant difference ( P < 0.05), n = 6. CON is the basal diet group; HDG is the 500 mg/kg HDCA diet group; LCG is the 500 mg/kg LCA diet group. ACC = acetyl-CoA-carboxylase; FABP3 = fatty acid binding protein 3; PPARG = peroxisome proliferator-activated receptor gamma; FXR = farnesoid X receptor; TGR5 = Takeda G-protein-coupled receptor 5; HSL = hormone-sensitive triglyceride lipase; CPT = Carnitine palmitoyltransferase; SREBP-1c = sterol regulatory element-binding protein-1c; CYP7A1 = cholesterol 7α-hydroxylase; CYP27A1 = sterol 27-hydroxylase; ChREBP = carbohydrate response element binding protein; FAS = fatty acid synthase; HDCA = hyodeoxycholic acid; LCA = lithocholic acid.

Article Snippet: The primary antibodies against HSL (4107, Cell Signaling Technology, Danvers, MA, USA), p-HSL (45804, Cell Signaling Technology, Danvers, MA, USA), phosphorylated acetyl-CoA-carboxylase (p-ACC) (42801, Cell Signaling Technology, Danvers, MA, USA), Takeda G-protein-coupled receptor 5 (TGR5) (ab72608, Abcam, UK), peroxisome proliferator activated receptor gamma (PPARG) (2443, Cell Signaling Technology, Danvers, MA, USA), β-actin (4967, Cell Signaling Technology, Danvers, MA, USA) and GAPDH (97166, Cell Signaling Technology, Danvers, MA, USA) were diluted to a concentration of 1:1000.

Techniques: Gene Expression, Binding Assay

Journal: Animal Nutrition

Article Title: Different types of bile acids exhibit opposite regulatory effects on lipid metabolism in finishing pigs through bile acid receptors

doi: 10.1016/j.aninu.2024.12.003

Figure Lengend Snippet: Effects of HDCA on the relative expressions of lipid metabolism-related factors in 3T3-L1 mature adipocytes. (A-B) Effects of HDCA on the levels of lipid metabolism-related gene expression; n = 6. (C-D) Relative protein expressions and grayscale analysis of lipid metabolism-related factors in HDCA-treated groups; n = 3. Values with different letters mean significant difference ( P < 0.05). ACC = acetyl-CoA-carboxylase; C/EBPα = enhancer binding proteins alpha; FXR = farnesoid X receptor; TGR5 = Takeda G-protein-coupled receptor 5; SREBP-1c = sterol regulatory element-binding protein-1c; FABP4 = fatty acid binding protein 4; PPARG = peroxisome proliferator-activated receptor gamma; HSL = hormone-sensitive triglyceride lipase; p-HSL = phosphorylated hormone-sensitive triglyceride lipase; p-ACC = phosphorylated acetyl-CoA-carboxylase; HDCA = hyodeoxycholic acid; GW4064 = the farnesoid X receptor agonist.

Article Snippet: The primary antibodies against HSL (4107, Cell Signaling Technology, Danvers, MA, USA), p-HSL (45804, Cell Signaling Technology, Danvers, MA, USA), phosphorylated acetyl-CoA-carboxylase (p-ACC) (42801, Cell Signaling Technology, Danvers, MA, USA), Takeda G-protein-coupled receptor 5 (TGR5) (ab72608, Abcam, UK), peroxisome proliferator activated receptor gamma (PPARG) (2443, Cell Signaling Technology, Danvers, MA, USA), β-actin (4967, Cell Signaling Technology, Danvers, MA, USA) and GAPDH (97166, Cell Signaling Technology, Danvers, MA, USA) were diluted to a concentration of 1:1000.

Techniques: Gene Expression, Binding Assay

Journal: Animal Nutrition

Article Title: Different types of bile acids exhibit opposite regulatory effects on lipid metabolism in finishing pigs through bile acid receptors

doi: 10.1016/j.aninu.2024.12.003

Figure Lengend Snippet: Fig. 5. Effects of HDCA on the relative expressions of lipid metabolism-related factors in 3T3-L1 mature adipocytes. (A-B) Effects of HDCA on the levels of lipid metabolism-related gene expression; n ¼ 6. (C-D) Relative protein expressions and grayscale analysis of lipid metabolism-related factors in HDCA-treated groups; n ¼ 3. Values with different letters mean significant difference (P < 0.05). ACC ¼ acetyl-CoA-carboxylase; C/EBPa ¼ enhancer binding proteins alpha; FXR ¼ farnesoid X receptor; TGR5 ¼ Takeda G-protein-coupled receptor 5; SREBP-1c ¼ sterol regulatory element-binding protein-1c; FABP4 ¼ fatty acid binding protein 4; PPARG ¼ peroxisome proliferator-activated receptor gamma; HSL ¼ hormone-sensitive triglyceride lipase; p-HSL ¼ phosphorylated hormone-sensitive triglyceride lipase; p-ACC ¼ phosphorylated acetyl-CoA-carboxylase; HDCA ¼ hyodeoxycholic acid; GW4064 ¼ the farnesoid X receptor agonist.

Article Snippet: The primary antibodies against HSL (4107, Cell Signaling Technology, Danvers, MA, USA), p-HSL (45804, Cell Signaling Technology, Danvers, MA, USA), phosphorylated acetyl-CoAcarboxylase (p-ACC) (42801, Cell Signaling Technology, Danvers, MA, USA), Takeda G-protein-coupled receptor 5 (TGR5) (ab72608, Abcam, UK), peroxisome proliferator activated receptor gamma (PPARG) (2443, Cell Signaling Technology, Danvers, MA, USA), bactin (4967, Cell Signaling Technology, Danvers, MA, USA) and GAPDH (97166, Cell Signaling Technology, Danvers, MA, USA) were diluted to a concentration of 1:1000.

Techniques: Gene Expression, Binding Assay

Journal: Journal of Obesity & Metabolic Syndrome

Article Title: Alpha-Lipoic Acid Induces Adipose Tissue Browning through AMP-Activated Protein Kinase Signaling in Vivo and in Vitro

doi: 10.7570/jomes23048

Figure Lengend Snippet: Effects of treatment with ALA on (A) plasma levels of irisin and protein expression and quantification of (B) phosphorylated AMP-activated protein kinase (p-AMPK), (C) phosphorylated acetyl-CoA carboxylase (p-ACC), and (D) liver kinase B1 (LKB1) in inguinal white adipose tissue of ovariectomized rats. Data are expressed as mean±standard error of the mean. * P <0.05 vs. Sham; † P <0.05 vs. ovariectomy (Ovx) (n=6–11). ALA2, alpha-lipoic acid treatment (200 mg/kg/day, gavage); ALA3, alpha-lipoic acid (300 mg/kg/day, gavage) for 8 weeks.

Article Snippet: Primary antibodies against phosphorylated AMPK (p-AMPK), AMPK, phosphorylated ACC (p-ACC), ACC, LKB1 (1:1,000, Cell Signaling Technology), and β-actin (1:5,000, Cell Signaling Technology) and UCP1, PRDM16, and CIDEA (1:1,000, Abcam) were incubated at 4 °C overnight.

Techniques: Clinical Proteomics, Expressing